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Buy a mosaic photo frame for birthday online in India. ![]() ![]() This session cookie is served by our membership/subscription system and controls whether you are able to see content which is only available to logged in users. The cookie is set by the GDPR Cookie Consent plugin and is used to store whether or not user has consented to the use of cookies. The cookie is a session cookies and is deleted when all the browser windows are closed. The cookie is used to store and identify a users' unique session ID for the purpose of managing user session on the website. This cookie is native to PHP applications. The cookie is used to remember the user consent for the cookies under the category "Performance". This cookie is set by GDPR Cookie Consent WordPress Plugin. The cookie is used to store the user consent for the cookies in the category "Necessary". This cookie is set by GDPR Cookie Consent plugin. The cookie is used to remember the user consent for the cookies under the category "Analytics". ![]() The cookie is set by GDPR cookie consent to record the user consent for the cookies in the category "Advertising & Targeting". CookieĬookielawinfo-checkbox-advertising-targeting These cookies do not store any personal information. This category only includes cookies that ensures basic functionalities and security features of the website. Necessary cookies are absolutely essential for the website to function properly. In 2019 1. 62 billion people rode the Shenzhen metro, earning it tenth spot on our list. ![]() A city with lofty financial ambitions like Shenzhen needs a metro system to match, and that’s what the Guangdong city has. Sitting on the Pearl River Delta and bordering Hong Kong to the South, Shenzhen is one of China’s biggest cities and ranks highly in the Global Financial Centres Index. One of the youngest systems on this list, the Shenzhen Metro began operating in 2004, yet it ranks as the fourth longest system in the world after several extensions (the latest of which was opened in 2020). While different transport authorities release their ridership statistics at different times, using the latest figures from each metro we have been able identify the top 10 busiest systems in the world. There are few systems, however, that can boast the ridership figures of the below systems (outside of COVID-19 restrictions of course). Almost every major city around the world possesses its own metro network, into which commuters pile into every day to get to and from work. Since the first underground railway opened in London in 1863 between Farringdon and Paddington, the metro has become one of the most important modes of mass transportation in the modern world. ![]() ![]() ![]() Additionally, Kakao would share user and sales-related data with participating online companies on Talk Channel 2.0. Kakao would also equip its new services with simple features, including seamless login and payment. However, Kakao took a different approach with Talk Channel 2.0 by charging zero commissions. Kakao’s fellow market competitors charge a commission rate to sellers that use their online shopping platforms. Moreover, Talk Channel 2.0, Kakao’s new e-commerce platform, would enable smaller companies to manage digital marketplaces using the KakaoTalk app. Due to its rising business concerns, Kundal took Kakao’s invitation to help foster its new online shopping services. Kundal recently expressed concern over its heightening dependency on other e-commerce companies, including Coupang and Naver, regarding its increasing revenue. Last year, only four years after its establishment, Kundal recorded a 120 billion won ($108 million) revenue. In May, a Kakao business unit, KakaoTalk Channel, collaborated with Kundal, a K-beauty startup that became an industry sensation. ![]() Retail industry sources also said that Kakao conducted trial operations with various companies, including Kundal, Korean Air Lines, and Nike. Kakao reached out to these companies, encouraging them to expand their digital shopping operations to Kakao’s new platform. Some of the companies Kakao consulted include LG Household & Healthcare, Maeil Dairies Co., Samsung Electronics Co., and Yuhan-Kimberly Ltd. Furthermore, retail industry sources stated that Kakao would introduce its e-commerce platform sometime in July.īefore revealing its new e-commerce platform launch, Kakao had collaborated with market-leading manufacturers with extensive know-how in their respective sectors. At present, KakaoTalk is South Korea’s most extensive messaging application. Kakao would also significantly cultivate the various online shopping companies on its existing services by adopting the previously mentioned measures.Īs part of its e-commerce venture, Kakao would incorporate accumulated data from more than 49 million monthly active KakaoTalk users. ![]() Notably, Kakao plans to charge zero commission rates and apply a direct-to-consumer (D2C) business model to its upcoming e-commerce platform. have been leading the domestic e-commerce market. ![]() In particular, Kakao would be launching its e-commerce platform during this year’s second half. currently prepares to participate in the South Korean e-commerce sector. South Korea’s music streaming and download market was worth an estimated $406 million in 2015, according to Seoul-based brokerage Hana Financial Investment.Kakao Corp. It will also give the operator of the country’s dominant chat app, KakaoTalk, a head-start on Swedish music streaming service Spotify, which has yet to launch here. The deal, Kakao’s biggest since its 2014 all-stock merger with web search operator Daum, gives the mobile chat company access to the biggest streaming service user base in South Korea as well as a library of popular music content. Leon also owns its own record label with “K-Pop” artists such as IU. The South Korean firm said it would buy a 76.4 percent stake in MelOn streaming service operator Loen Entertainment Inc for $1.6 billion. SEOUL (Reuters) - Chat app operator Kakao Corp on Monday unveiled a $1.6 billion (1.9 trillion won) deal to buy control of South Korea’s top music streaming service provider, tapping the market for Korean pop to diversify revenues and even expand offshore. ![]() ![]() ![]() The data are expressed as the percentage of invasion. The stained nuclei on 6 randomly selected microscopic fields for each sample were counted, and the quantification of migrated cells was done in 3 independent experiments. The cells that migrated to the lower side of the filter were then fixed with 100% methanol for 2 minutes and stained with 0.1% crystal violet. Noninvading or nonmigrating cells were removed from the upper side of the Matrigel chamber by gently but firmly swabbing with cotton tips. The chambers were then incubated for 24 hours at 37☌, in 5% CO 2. The same number of cells was also seeded in the control chamber containing an identical polycarbonate membrane filter without Matrigel, for free migration and viability testing. The bottom chamber was filled with MEM supplemented with 10% FBA. Briefly, following transfection, 5 × 10 4 cells were added to the top chamber containing a polycarbonate membrane filter of 8-μm pore size with rehydrated Matrigel. Materials and MethodsĬellular invasion into Matrigel was done with the BD BioCoat Matrigel Invasion Chamber (BD Biosciences). As the tumor progresses and p53 activity is lost, MnSOD levels increase again, creating conditions in which cancer cells can survive under oxidative stress. When a tumor begins to develop, the modulation of stress-sensitive transcription factors, including p53, creates an environment of high oxidative stress achieved by the suppression of MnSOD. The data support a model in which activation of p53 by carcinogens leads to a reduction in MnSOD that precedes the appearance of cancer. The loss of Sp1 function exacerbates p53 siRNA–mediated cell transformation but not invasion. Using siRNA-knockdown approaches coupled with in vitro tumor invasion assay, we also show that suppression of p53 alone led to increased invasion. Here, we evaluated changes in MnSOD expression during the course of tumorigenesis and show that MnSOD expression was suppressed in early tumorigenic stages but was restored later in advanced carcinoma. ![]() p53 is mutated in at least 50% of tumors, and the wild-type p53 tumor suppressor is completely lost in many tumor tissues ( 18, 19). P53 is a transcriptional regulator with tumor suppression capability. These findings also provide strong support for the development of means to reactivate p53 for the prevention of tumor progression. ![]() The results identify MnSOD as a p53-regulated gene that switches between early and advanced stages of cancer. We used chromatin immunoprecipitation, electrophoretic mobility shift assay, and both knockdown and overexpression of Sp1 and p53 to verify their roles in the expression of MnSOD at each stage of cancer development. In squamous cell carcinomas, Sp1 binding increased because of the loss of functional p53. Exposure to DMBA and TPA activated p53 and decreased MnSOD expression via p53-mediated suppression of Sp1 binding to the MnSOD promoter in normal-appearing skin and benign papillomas. The suppression and subsequent restoration of MnSOD expression were mediated by two transcription-factors, Sp1 and p53. The results show that MnSOD expression was suppressed at a very early stage but increased at late stages of skin carcinogenesis. Here, we generated transgenic mice expressing a luciferase reporter gene under the control of human MnSOD promoter-enhancer elements and investigated the changes of MnSOD transcription using the 7,12-dimethylbenz(α)anthracene (DMBA)/12- O-tetradecanoylphorbol-l3-acetate (TPA) multistage skin carcinogenesis model. However, despite extensive studies in MnSOD regulation and its role in cancer, when and how the alteration of MnSOD expression occurs during the process of tumor development in vivo are unknown. Manganese superoxide dismutase (MnSOD) plays a critical role in the survival of aerobic life, and its aberrant expression has been implicated in carcinogenesis and tumor resistance to therapy. ![]() ![]() Aim to get about 150 minutes of aerobic exercise each week. Exercise increases blood flow to your body, including your brain. It can interfere with the memory-encoding process. Multitasking naturally divides your attention. ![]() If you’re trying to commit something to your long-term memory, try recalling it just before falling asleep. Sleep is important for consolidating your memories so you can recall them later. Want to fine-tune your memory to be as efficient as possible? The following tips may help to boost your long-term memory and prevent memory loss: Sometimes, retrieval happens effortlessly. This usually happens in response to retrieval cues, or things that trigger you to search for a memory.įor example, if someone asks you a trivia question, that’s your retrieval cue to search your memory for specific information. Retrieval is the process of recalling information that’s been encoded and stored as a memory. For example, the visual parts of the memory are stored in the area of the brain associated with vision. In storage, memories can be maintained for longer periods of time.Ī single long-term memory can be stored in many parts of your brain. Once a memory has been encoded, it’s ready to be stored in your brain. The level of processing that occurs can vary from shallow (focusing on physical features, color, or size) to deep (focusing on the meaning of the item or its relationship to other things). From there, the information enters your memory. ![]() Step 1: EncodingĪt this stage, your senses take information from your environment and send it to your brain. Long-term memories, including explicit memories, are made over the course of three steps. ![]() ![]() ![]() The Post has since taken down the online animated cartoon, by Pulitzer Prize winner Ann Telnaes, which depicted Cruz holding two dancing monkeys on leashes. His daughters Caroline and Catherine are 7 and 5, respectively. ![]() "Not much ticks me off, but making fun of my girls, that will do it," Cruz told reporters in Tulsa, Okla. Ted Cruz on Wednesday blasted a Washington Post editorial cartoon that portrayed his two young daughters as monkeys on leashes, stating that children should be off-limits. If we still care about why everyone is mad, let's go to WaPo itself. "Do you know why Chelsea Clinton is so ugly?" Still don't know what the dastardly WaPo did to the Cruz kids, besides possibly expose them to their website that loads worse than Wonkette times Salon, but I feel like there is some kind of memory niggling at my brain, let me put down the opium pipe and remember REAL HARD. Like, way more of a dick move than when Tammy ran for president against her brother, the hot guy from American Pie, because they both wanted to bang the same chick. But can she clarify?įirstly, I had no idea Chelsea Clinton was running for president, which seems like a real dick move at her mom. OK, so the Washington Post said something about Ted Cruz's kids. Let's see if Meghan McCain can explain it to us: ![]() ANYWAY, why won't everyone Think of the Children today? Let's find out together! Oh, wait, isn't it still Ted Cruz's first term in the Senate? Is the Oval Office really where Republicans want their leaders to get "on the job experience"? LOL silly, that is only if the president is black. Which is weird, because we were calling them jerk babies back when Dad was just getting sworn in to his first term in the Senate. ![]() Favorites such as “How Obamacare Stole Christmas” and “Rudolph the Underemployed Reindeer.We guess someone said something mean about Ted Cruz's kids, and it was not Wonkette. … A Proven Record presents a collection of timely Christmas classics read by the trusted conservative leader, Ted Cruz. Imagine the greatest Christmas stories, told by the senator who once read “Green Eggs and Ham” from the Senate floor. Telnaes defended her cartoon in the original posting of it, writing, “When a politician uses his children as political props, as Ted Cruz recently did in his Christmas parody video in which his eldest daughter read (with her father’s dramatic flourish) a passage of an edited Christmas classic, then I figure they are fair game,” adding later on Twitter, “Ted Cruz has put his children in a political ad - don’t start screaming when editorial cartoonists draw them as well.”Ĭruz had released an ad showing himself reading his daughters stories called, “How Obamacare Stole Christmas” and “Rudolph the Underemployed Reindeer.” In the video, which was released during Cruz’s presidential campaign, the narrator stated: Stick w/ attacking me–Caroline & Catherine are out of your league. Stick w/attacking me – Caroline and Catherine are out of your league.”Ĭlassy. 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Of course, the virtual reality gear we'll all have strapped to our heads 15 years from now probably won't be as bulky or ugly as the headsets people are using today. Today, of course, most Americans use their camera-equipped, internet-connected smartphones many times every day. I hope the next version will improve the picture resolution to at least 1080p. The Daily Show's Jon Stewart mocked the idea of cellphones with cameras in them in 2004. Mark Zuckerberg announces our first Standalone VR headset, Oculus Go. Lots of smart people thought the internet was a joke 20 years ago. Virtual reality is such a promising technology that I'd bet money that within the next 15 years, most people reading this article will spend many hours with some kind of virtual reality goggles strapped to their face, watching 3D movies, playing 3D video games, or consuming forms of media that are just being invented now.Īnd this won't be the first time new technologies are widely mocked a decade before they become indispensable. ![]() I've talked to several people who have had a chance to try out Facebook's Oculus Rift, and every one of them was blown away by the experience. People described the photo as dystopian - and obviously there is something a little bit creepy about a room full of people totally ignoring each other.īut the internet's collective freakout misses an important point: No matter how ridiculous it might look to us today, there's every reason to think virtual reality is going to be a big deal in the next few years. When Mark Zuckerberg posted this photo of himself walking past an auditorium full of people wearing virtual reality headsets, it provoked predictable mockery on Twitter. ![]() |